ABSTRACT
BACKGROUND: Studies show that proper exercise has the effect of anti-aging, increasing bone mass accumulation and reducing bone mass loss. OBJECTIVE: To investigate the effect of aerobic exercises on osseous composition In aging rats. DESIGN, TIME AND SETTING: A randomized control animal experiment was completed in the Animal Experiment Laboratory of Xi'an Sports Institute from April to June in 2007. MATERIALS: A total of 64 clean male SD rats weaned for 2 months were randomly divided into a normal control group, a non-modeling exercise group, an aging model group and a modeling exercise group, with 16 ones in each group. Every 8 rats of each group were sacrificed at the 6th and 9th week respectively.exercise for 6 and 9 weeks. MAIN OUTCOME MEASURES: At the end of week 6 and 9, rats in each group were detected regarding to the following measures: serum alkaline phosphatase, bone gla protein, serum propeptide of type] procollagen, serum total calcium, serum inorganic phosphorus, serum magnesium, urinary calcium, urinary inorganic phosphorus, urinary magnesium, urinary hydroxylysine glycoside. Bone trabecula changes in femoral bone tissue were observed with light microscope. RESULTS: Compared with the normal control group, the aging model group got a decrease at the following indexes at both week 6 and week 9, namely, serum alkaline phosphatase, serum propeptide of type Ⅰ procellagen, serum total calcium, serum magnesium, urinary calcium, urinary inorganic phosphorus, urinary hydroxyproline. While in the non-modeling exercise group and the modeling exercise group, these above-mentioned indexes increased. Compared with the normal control group, the aging model group got an increase at the following indexes at both week 6 and week 9, namely, serum osteocalcin, inorganic phosphorus, adnary magnesium, urinary hydroxylysine glycoside. While in the non-modeling exercise group and the modeling exercise group, these above-mentioned indexes decreased. In Addition, exercise of 9 weeks led to a better recovery result than that of 6 weeks. The detection under light microscope showed an obvious few bone trabeculas in the aging model group and an increase of bone trabeculas per unit volume in the non-modeling exercise group and the modeling exercise group. CONCLUSION: Aerobic exercise has a great effect on the morphosis of femoral bone osseous composition in aging rats, and it can delay osteoporosis.
ABSTRACT
Objective To evaluate the effect of tranilast on chronic cyclosporin A(CsA)nephrotoxicity rats and the possible mechanisms of protective effect of it.Methods Rats were on low salt diet and CsA was administered by gastric gavage at a dose of 20 mg/(kg?d)for 28 days,and tranilast was gave to these rats in a dose of 400 mg/(kg?d).The renal function and renal morphology were observed at1,2 and 4 weeks after administration of tranilast.Results CsA induced the corruption of normal condition and weight lost were markedly delayed after administration of tranilast.On histopathology tranilast attenuated tubular cells vacuolations as well as interstitial damage including interstitial ED1+ macrophages infiltration and focal interstitial fibrosis.Meanwhile,tranilast down-regulated the protein of OPN in kidney.Conclusion Tranilast can attenuate the structural changes of kidney in chronic CsA nephrotoxicity rats.Decreased CsA-related ED1+ macrophages,OPN up-regulation expression in the kidney may be related to mechanisms of protective effect of tranilast in the treatment of CsA induced chronic nephrotoxicity.